Difference between revisions of "Part:BBa K2305016"
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We calculated the new part’s base number to be 2417bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct. | We calculated the new part’s base number to be 2417bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct. | ||
[[Image:BIT Figure DNA sequencing K2305016.png|200px|left|K230500]]<br clear="all"> | [[Image:BIT Figure DNA sequencing K2305016.png|200px|left|K230500]]<br clear="all"> | ||
| + | ==Characterization map== | ||
| + | [[Image:BIT Figure map K2305016.png|400px|left|K230500]]<br clear="all"> | ||
| + | Fig.The Growth curve of E.coil. | ||
| + | [[Image:BIT Figure map K2305016a.png|400px|left|K230500]]<br clear="all"> | ||
| + | Fig.The GFP curve of E.coil. | ||
Latest revision as of 02:16, 28 October 2017
GFP with cycle amplification system
Plux promoter stars the circuit, and Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux and form a cyclic amplifier to achieve a higher GFP expression.
Agarose gel electrophoresis
We calculated the new part’s base number to be 2417bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
Characterization map
Fig.The Growth curve of E.coil.
Fig.The GFP curve of E.coil.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1525
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2204