Difference between revisions of "Part:BBa K2305011"
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==DNA sequencing== | ==DNA sequencing== | ||
The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further | The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further | ||
| − | [[Image:BIT Figure DNA sequencing K2305011a.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011b.png|200px|left|K230500]]<br clear="all"> | + | [[Image:BIT Figure DNA sequencing K2305011a.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011b.png|200px|left|K230500]] |
| + | [[Image:BIT Figure DNA sequencing K2305011c.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011d.png|200px|left|K230500]]<br clear="all"> | ||
Revision as of 19:05, 27 October 2017
GFP/RFP dual fluorescent system with pbad
GFP/RFP dual fluorescent system with pbad
Agarose gel electrophoresis
We calculated the new part’s base number to be 4352bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
DNA sequencing
The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2383
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 4064
Illegal AgeI site found at 4176 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3062
Illegal SapI site found at 961