Difference between revisions of "Part:BBa K2360000:Design"
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===References=== | ===References=== | ||
| + | [1]. Kloos D U, Strätz M, Güttler A, et al. Inducible cell lysis system for the study of natural transformation and environmental fate of DNA released by cell death.[J]. 1994, 176(23):7352-7361. | ||
Latest revision as of 18:48, 27 October 2017
SRRz+rrnb terminater
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The SRRz gene codes maybe three protein S,R,Rz.The product of S gene would cause lesions on the cytoplasmic membrane through which the product coded by the R gene escapes to the periplasm and causes murein-degrading, while the Rz gene’s product may be an endopeptidase that can cleave the oligopeptide crosslinks in the peptidoglycan and/or between peptidoglycan and the outer membrane. The expression of this gene finally cause the lysis of E.coil. We use it as a lysis module to make a high-efficiency biosensor.
Source
The gene is come from phage.
References
[1]. Kloos D U, Strätz M, Güttler A, et al. Inducible cell lysis system for the study of natural transformation and environmental fate of DNA released by cell death.[J]. 1994, 176(23):7352-7361.