Difference between revisions of "Part:BBa K2305008"
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The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
 
The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
 
[[Image:BIT Figure DNA sequencing K2305008a.png|200px|left|K230500]] <br clear="all">
 
[[Image:BIT Figure DNA sequencing K2305008a.png|200px|left|K230500]] <br clear="all">
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==Characterization map==
 +
[[ImageBIT Figure map K2305008.png|400px|left|K230500]]<br clear="all">
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Fig.The Growth curve of E.coil.
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Revision as of 22:19, 27 October 2017


pcat promoter and plux,luxr generator

pcat promtor controlled plux and LuxR

Agarose gel electrophoresis

We calculated the new part’s base number to be 928bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500

DNA sequencing

The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

K230500

Characterization map

400px|left|K230500
Fig.The Growth curve of E.coil.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 64