Difference between revisions of "Part:BBa K2271104:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The pex13 was used as a peroxisomal membrane anchor for fluorescence proteins.
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The membrane domain PEX13 (amino acids 200-310) was used as a peroxisomal membrane anchor for fluorescence proteins. We therefore added a triple GGGGS linker to allow an undisturbed c-terminal fusion.
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+
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===Source===
 
===Source===

Revision as of 12:12, 28 October 2017


PEX13 membrane anchor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The membrane domain PEX13 (amino acids 200-310) was used as a peroxisomal membrane anchor for fluorescence proteins. We therefore added a triple GGGGS linker to allow an undisturbed c-terminal fusion.

Source

UniProtKB - P80667 (PEX13_YEAST)

References