Difference between revisions of "Part:BBa K2271118"

Revision as of 15:32, 30 October 2017

ValS

Valencene synthase converts FPP into Valencene. We chose the ValS from Callitropis nootkatensis, because other valencene synthases perform relatively inefficient in microorganisms. For example, they show a relatively poor product specificity, are less efficient in producing sesquiterpene than other synthases and have a significant amount of side products like germacrene A. In contrast to this, ValS from C. nootkatensis has a good product specificity and is more robust in terms of pH and temperature changes. It has also a better yield than citrus valencene synthases in yeast.

This part is designed for cytosolic expression, for peroxisomal import please choose part:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal suffix found in sequence at 2732
Illegal BglII site found at 860
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2271118 short</partinfo>
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+Valencene synthase converts FPP into Valencene.
+We chose the ValS from Callitropis nootkatensis, because other valencene synthases perform relatively inefficient in microorganisms.  For example, they show a relatively poor product specificity, are less efficient in producing sesquiterpene than other synthases and have a significant amount of side products like germacrene A.
+In contrast to this, ValS from C. nootkatensis has a good product specificity and is more robust in terms of pH and temperature changes. It has also a better yield than citrus valencene synthases in yeast.
+This part is designed for cytosolic expression, for peroxisomal import please choose part:
 <!-- Add more about the biology of this part here