Difference between revisions of "Part:BBa I742157:Experience"
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| + | ; Characterization of crtZ | ||
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| − | <partinfo> | + | <partinfo>BBa_I742158 AddReview number</partinfo> |
| − | <I> | + | <I>Tokyo Tech iGEM2010</I> |
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| − | + | Our team synthesized zeaxanthin by several kinds of construct to confirm the activity of BioBrick parts designed by Edinburgh 2007. By assembling the ''crtZ'', ''crtEBIY'' and appropriate promoters, we constructed new BioBrick parts to synthesize zeaxanthin and compared their function . (→[https://parts.igem.org/wiki/index.php?title=Part:BBa_K395701 BBa_K395701], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K395702 BBa_K395702], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K395704 BBa_K395704])<br><br> | |
| + | We constructed two types of zeaxanthin synthetic construct. One is that ''crtZ''(BBa_I742158) and ''crtEBIY''(BBa_K274210) were assembled on a single plasmid.(We call this construct “single plasmid construct”.)The other is that ''crtZ'' and ''crtEBIY''were assembled on different plasmids.(we call this type of constructs “double plasmids construct”.) | ||
| + | We made 5 combinations shown in table<1>.<br><br> | ||
| + | Constructs containing pBad/araC promoter on high-copy vector show the best pruduction and double plasmids construct shows as much pruduction as single plasmid construct under same promoter conbination. Construct with pBad/araC promoter shows better production than that with plac promoter. Construct that ''crtEBIY'' on high-copy vector and ''crtZ'' on low-copy vector shows as much expression as construct that ''crtEBIY'' on low-copy vector and ''crtZ'' on high-copy vector.（Actually, ③ showed a little more production than ⑤.)<br><br> | ||
| + | [[Image:tokyotech_Fig. 1.1.7.1.BBa_K395704.jpg|right|300px|thumb|figure1]] | ||
| + | [[Image:tokyotech_Fig. 1.1.8.1. zeaxanthin pellet and plate.jpg|left|300px|Fig. 1.1.8.1. zeaxanthin pellet and plate]] | ||
| + | [[Image:tokyotech_table. 1.1.1.2.jpg|300px|left|table<1>]] | ||
|}; | |}; | ||
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Revision as of 21:58, 27 October 2010
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