Difference between revisions of "Part:BBa K2205004"
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[[File:T--Newcastle--Correct sox protein gel.png|400px|]]
 
[[File:T--Newcastle--Correct sox protein gel.png|400px|]]
  
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'''Sarcosine Oxidase Expression:'''Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: ,2 ,3 ,4 ,5 ,6 ,7 ,8 ,9 ,10 .
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'''Sarcosine Oxidase Expression:'''Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: 6 µl ladder, 2: 10 µl sfGFP, 3: BL21-DE3, 4: 10µl SOX 1, 5: 10 µl SOX 2, 6: 10 µl SOX 3, 7: 10 µl SOX 4, 8: 10 µl SOX 5, 9: 10 µl SOX 6, 10: 6 µl ladder.
  
  

Revision as of 18:36, 27 October 2017


T7-Sarcosine Oxidase

Sarcosine Oxidase under the control of an IPTG-inducible T7 promoter. Catalyses sarcosine to yield glycine, formaldehyde and hydrogen peroxide.

Induction with IPTG

T--Newcastle--Correct sox protein gel.png

Sarcosine Oxidase Expression:Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: 6 µl ladder, 2: 10 µl sfGFP, 3: BL21-DE3, 4: 10µl SOX 1, 5: 10 µl SOX 2, 6: 10 µl SOX 3, 7: 10 µl SOX 4, 8: 10 µl SOX 5, 9: 10 µl SOX 6, 10: 6 µl ladder.


Formaldehyde Production

T--Newcastle--SOX testing.JPG

Testing of Sarcosine Oxidase with Sarcosine: Lysed cell cultures induced with 40 µl of a 100 mM stock of IPTG tested with formaldehyde strips. From left to right: SOX+0 µl sarcosine, SOX+IPTG+0 µl sarcosine, WT+0 µl sarcosine, WT+IPTG+0 µl sarcosine, SOX+50 µl sarcosine, SOX+IPTG+50 µl sarcosine, WT+50 µl sarcosine, WT+IPTG+50 µl sarcosine, SOX+200 µl sarcosine, SOX+IPTG+200 µl, WT+200 µl sarcosine, WT+IPTG+200 µl.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]