Difference between revisions of "Part:BBa K2205004"
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[[File:T--Newcastle--Correct sox protein gel.png|400px|]] | [[File:T--Newcastle--Correct sox protein gel.png|400px|]] | ||
| − | '''Sarcosine Oxidase Expression:'''Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: | + | '''Sarcosine Oxidase Expression:'''Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: ,2 ,3 ,4 ,5 ,6 ,7 ,8 ,9 ,10 . |
Revision as of 17:41, 27 October 2017
T7-Sarcosine Oxidase
Sarcosine Oxidase under the control of an IPTG-inducible T7 promoter. Catalyses sarcosine to yield glycine, formaldehyde and hydrogen peroxide.
Induction with IPTG
Sarcosine Oxidase Expression:Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: ,2 ,3 ,4 ,5 ,6 ,7 ,8 ,9 ,10 .
Formaldehyde Production
Testing of Sarcosine Oxidase with Sarcosine: Lysed cell cultures induced with 40 µl of a 100 mM stock of IPTG tested with formaldehyde strips. From left to right: SOX+0 µl sarcosine, SOX+IPTG+0 µl sarcosine, WT+0 µl sarcosine, WT+IPTG+0 µl sarcosine, SOX+50 µl sarcosine, SOX+IPTG+50 µl sarcosine, WT+50 µl sarcosine, WT+IPTG+50 µl sarcosine, SOX+200 µl sarcosine, SOX+IPTG+200 µl, WT+200 µl sarcosine, WT+IPTG+200 µl.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]