Difference between revisions of "Part:BBa K2205004"
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[[File:T--Newcastle--Correct sox protein gel.png|400px|]]
 
[[File:T--Newcastle--Correct sox protein gel.png|400px|]]
  
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'''Sarcosine Oxidase Expression:'''Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG.
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'''Sarcosine Oxidase Expression:'''Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: ;2 ;3 ;4 ;5
  
  

Revision as of 17:41, 27 October 2017


T7-Sarcosine Oxidase

Sarcosine Oxidase under the control of an IPTG-inducible T7 promoter. Catalyses sarcosine to yield glycine, formaldehyde and hydrogen peroxide.

Induction with IPTG

T--Newcastle--Correct sox protein gel.png

Sarcosine Oxidase Expression:Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: ;2 ;3 ;4 ;5


Formaldehyde Production

T--Newcastle--SOX testing.JPG

Testing of Sarcosine Oxidase with Sarcosine: Lysed cell cultures induced with 40 µl of a 100 mM stock of IPTG tested with formaldehyde strips. From left to right: SOX+0 µl sarcosine, SOX+IPTG+0 µl sarcosine, WT+0 µl sarcosine, WT+IPTG+0 µl sarcosine, SOX+50 µl sarcosine, SOX+IPTG+50 µl sarcosine, WT+50 µl sarcosine, WT+IPTG+50 µl sarcosine, SOX+200 µl sarcosine, SOX+IPTG+200 µl, WT+200 µl sarcosine, WT+IPTG+200 µl.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]