Difference between revisions of "Part:BBa K2205005"
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The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional. The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to <i>rhlI</i> (J64718). This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.<br/><br/> | The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional. The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to <i>rhlI</i> (J64718). This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.<br/><br/> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2017/2/2d/--T--Newcastle--MP--Expression_of_GFP_Fim.png"/> | |
Expression of sfGFP over a standard growth curve. The Fim switch strain was co-cultured with the reporter strain (K2205014) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL. FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL. Each data point it the mean of 3 biological replicates.<br/><br/> | Expression of sfGFP over a standard growth curve. The Fim switch strain was co-cultured with the reporter strain (K2205014) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL. FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL. Each data point it the mean of 3 biological replicates.<br/><br/> | ||
Revision as of 17:32, 27 October 2017
Fim Standby Switch
The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional. The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to rhlI (J64718). This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.
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Expression of sfGFP over a standard growth curve. The Fim switch strain was co-cultured with the reporter strain (K2205014) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL. FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL. Each data point it the mean of 3 biological replicates.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]