Difference between revisions of "Part:BBa K2205004"

Revision as of 17:37, 27 October 2017

T7-Sarcosine Oxidase

Sarcosine Oxidase under the control of an IPTG-inducible T7 promoter. Catalyses sarcosine to yield glycine, formaldehyde and hydrogen peroxide.

IPTG Induction

Formaldehyde Production

Testing of Sarcosine Oxidase with Sarcosine: Lysed cell cultures induced with 40 µl of a 100 mM stock of IPTG tested with formaldehyde strips. From left to right: SOX+0 µl sarcosine, SOX+IPTG+0 µl sarcosine, WT+0 µl sarcosine, WT+IPTG+0 µl sarcosine, SOX+50 µl sarcosine, SOX+IPTG+50 µl sarcosine, WT+50 µl sarcosine, WT+IPTG+50 µl sarcosine, SOX+200 µl sarcosine, SOX+IPTG+200 µl, WT+200 µl sarcosine, WT+IPTG+200 µl.

Sequence and Features