Difference between revisions of "Part:BBa K2382012"
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<partinfo>BBa_K2382012 short</partinfo>
 
<partinfo>BBa_K2382012 short</partinfo>
  
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==Usage and Biology==
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===<span class='h3bb'>Sequence and Features</span>===
 
===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K2382001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2382001 SequenceAndFeatures</partinfo>
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===Short Description===
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===Usage and Biology===
 
A rigid linker link two proteins to form one fusion protein. It can prevent the
 
A rigid linker link two proteins to form one fusion protein. It can prevent the
 
two proteins from interrupting each other while folding.
 
two proteins from interrupting each other while folding.
  
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===Usage and Biology===
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The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the
 
The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the
 
target protein becomes automatically released from the fusion protein without proteolytic treatment.
 
target protein becomes automatically released from the fusion protein without proteolytic treatment.
 
Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has
 
Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has
 
been successfully employed for many cases of protein purification without the tag of a fusion protein.
 
been successfully employed for many cases of protein purification without the tag of a fusion protein.
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===Characterization of the EAAAK===
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===References===
 
===References===
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Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated
 
Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated
 
EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021
 
EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021

Revision as of 12:47, 29 October 2017

EAAAK



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

A rigid linker link two proteins to form one fusion protein. It can prevent the two proteins from interrupting each other while folding.


The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the target protein becomes automatically released from the fusion protein without proteolytic treatment. Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has been successfully employed for many cases of protein purification without the tag of a fusion protein.

Characterization of the EAAAK

References

Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021