Difference between revisions of "Part:BBa K2325102"
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<b>Figure 1. Growth curve of <i>E.coli</i> BL21(DE3) and the engineering <i>E.coli</i> BL21(DE3)(pGEX-ptx) expressing phosphite dehydrogenase in a medium contains Na<sub>2</sub>HPO<sub>3</sub>•5H<sub>2</sub>0 (1.32mM) and NH<sub>4</sub>Cl serving as the phosphorus and nitrogen sources, respectively.</b> | <b>Figure 1. Growth curve of <i>E.coli</i> BL21(DE3) and the engineering <i>E.coli</i> BL21(DE3)(pGEX-ptx) expressing phosphite dehydrogenase in a medium contains Na<sub>2</sub>HPO<sub>3</sub>•5H<sub>2</sub>0 (1.32mM) and NH<sub>4</sub>Cl serving as the phosphorus and nitrogen sources, respectively.</b> | ||
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| − | The <i>E.coli</i> expressing phosphite dehydrogenase outcompeted a control strain in phosphite-containing media after 72 hours. | + | The <i>E.coli</i> expressing phosphite dehydrogenase outcompeted a control strain in phosphite-containing media after 72 hours. However, the negative control group still grew in a certain degree. We looked up the researches and presumed that, alkaline phosphatase(BAP) found in <i>E.coli</i>, will be increasingly express when the amount of bio-available phosphorus is low. Through the hydrolysis, the BAP then turn the phosphorus compounds in the environment into bio-available phosphorus. With the help of the BAP, the microbes can utilize the phosphorus compounds more effectively, which also facilitates the modified pathways. |
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Revision as of 03:28, 28 October 2017
Phosphite dehydrogenase expression device
The BBa_K2325101 contains formamidase gene with T7 promoter and terminator.
Usage and Biology
Figure 1. Growth curve of E.coli BL21(DE3) and the engineering E.coli BL21(DE3)(pGEX-ptx) expressing phosphite dehydrogenase in a medium contains Na2HPO3•5H20 (1.32mM) and NH4Cl serving as the phosphorus and nitrogen sources, respectively.
The E.coli expressing phosphite dehydrogenase outcompeted a control strain in phosphite-containing media after 72 hours. However, the negative control group still grew in a certain degree. We looked up the researches and presumed that, alkaline phosphatase(BAP) found in E.coli, will be increasingly express when the amount of bio-available phosphorus is low. Through the hydrolysis, the BAP then turn the phosphorus compounds in the environment into bio-available phosphorus. With the help of the BAP, the microbes can utilize the phosphorus compounds more effectively, which also facilitates the modified pathways.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]