Difference between revisions of "Part:BBa B0030:Experience"
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Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA (<em> E. coli</em> TOP10, high copy number plasmid) or LuxI: | Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA (<em> E. coli</em> TOP10, high copy number plasmid) or LuxI: | ||
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| + | WHU-iGEM 2017 | ||
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| + | https://parts.igem.org/Part:BBa_K2462006 | ||
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| + | We improved this RBS by adding a promoter function in Bacillus mageterium. | ||
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Revision as of 15:35, 27 October 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Characterizations of BBa_B0030
The Alverno_Ca team characterized BBa_K2066046, which includes BBa_B0030, as well as a series of other plasmids using TX-TL, a mean of in vitro transcription and translation using cell extract. Below is a graph of normalized expression of BBa_K2066046 in comparison to the other palsmids in the sequence.
This graph shows the fluorescence of the plasmids, over a period of 12 and a half hours, in a plate reader.
"
Applications of BBa_B0030
William and Mary iGEM 2016
We characterized the strengths of the Anderson RBS library.
User Reviews
UNIQ2b995b27c9d8210a-partinfo-00000000-QINU
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
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Aberdeen_Scotland 2009 |
The miniprep, single and double digests all worked. However we did not use this part for further cloning. |
UNIQ2b995b27c9d8210a-partinfo-00000003-QINU
UNIQ2b995b27c9d8210a-partinfo-00000004-QINU
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UNIPV-Pavia iGEM 2011 |
NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.
Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA ( E. coli TOP10, high copy number plasmid) or LuxI: WHU-iGEM 2017 https://parts.igem.org/Part:BBa_K2462006 We improved this RBS by adding a promoter function in Bacillus mageterium.
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UNIQ2b995b27c9d8210a-partinfo-00000007-QINU