Difference between revisions of "Part:BBa K2360008"

 
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<partinfo>BBa_K2360008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2360008 SequenceAndFeatures</partinfo>
  
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Figure 1. Bio-circuit of BBa_
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Our device has an obvious contact to mercury ions’ concentration.we measured the growth curve of our engineering bacteria in different concentration of mercury ions to prove its function.
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Figure 2. the growth curve of mercury detecting device in different concentration of mercury ions.
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Our device has an obvious contact to mercury ions’ concentration. By detecting the shading of the product’s color, we can qualitatively detect the concentration of mercury metals.
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Figure 3. the comparison between ONPG and X-gal. a. mercury detecting device’s chromogenic reaction at 50mins.
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We verified the detecting devices specificity of different metal ions.The result indicated that the mercury detecting device has good specificity for specific metal ions.
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Figure  4. The growth of the E. coli with lead detecting device after adding different metal ions and ddH<sub>2</sub>O
  
 
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Revision as of 00:00, 28 October 2017


rrnb terminater+MerR+pMerR +SRRz+rrnb terminater

It is a high-efficiency mercury detecting device. This device consist two parts, the lysis module (BBa_K2360000) and mercury detecting module(BBa_K2360003).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the mercury ion.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Figure 1. Bio-circuit of BBa_

Our device has an obvious contact to mercury ions’ concentration.we measured the growth curve of our engineering bacteria in different concentration of mercury ions to prove its function.

Figure 2. the growth curve of mercury detecting device in different concentration of mercury ions.

Our device has an obvious contact to mercury ions’ concentration. By detecting the shading of the product’s color, we can qualitatively detect the concentration of mercury metals.

Figure 3. the comparison between ONPG and X-gal. a. mercury detecting device’s chromogenic reaction at 50mins.

We verified the detecting devices specificity of different metal ions.The result indicated that the mercury detecting device has good specificity for specific metal ions.

Figure 4. The growth of the E. coli with lead detecting device after adding different metal ions and ddH2O