Difference between revisions of "Part:BBa K2268004"

 
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LacZ alpha fragment can associate with the omega fragment, if present, to restore beta-galactosidase activity to a cell. This can be visualized as a blue pigment color when cells are grown in the presence of X-gal.
 
LacZ alpha fragment can associate with the omega fragment, if present, to restore beta-galactosidase activity to a cell. This can be visualized as a blue pigment color when cells are grown in the presence of X-gal.
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Note: the Sequence diagram below does not reflect the promoter, BBa_K2268001, that drives expression of the cI repressor (K2268000) for technical reasons.
  
 
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Revision as of 16:18, 27 October 2017


p(cI)-LacZ alpha, [p(Lac)-cI] reverse

This is the LacZ alpha fragment (BBa_I732006) under the control of a cI responsive promoter (BBa_R0051), followed by a reverse complement copy of the cI repressor (BBa_K2268000) under the control of a LacI responsive promoter (BBa_K2268001). The latter two components act as an inverter: addition of IPTG will repress expression of the LacZ reporter, while a lack of IPTG will lead to expression.

LacZ alpha fragment can associate with the omega fragment, if present, to restore beta-galactosidase activity to a cell. This can be visualized as a blue pigment color when cells are grown in the presence of X-gal.

Note: the Sequence diagram below does not reflect the promoter, BBa_K2268001, that drives expression of the cI repressor (K2268000) for technical reasons.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]