Difference between revisions of "Part:BBa K2268004"
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LacZ alpha fragment can associate with the omega fragment, if present, to restore beta-galactosidase activity to a cell. This can be visualized as a blue pigment color when cells are grown in the presence of X-gal. | LacZ alpha fragment can associate with the omega fragment, if present, to restore beta-galactosidase activity to a cell. This can be visualized as a blue pigment color when cells are grown in the presence of X-gal. | ||
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+ | Note: the Sequence diagram below does not reflect the promoter, BBa_K2268001, that drives expression of the cI repressor (K2268000) for technical reasons. | ||
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Revision as of 16:18, 27 October 2017
p(cI)-LacZ alpha, [p(Lac)-cI] reverse
This is the LacZ alpha fragment (BBa_I732006) under the control of a cI responsive promoter (BBa_R0051), followed by a reverse complement copy of the cI repressor (BBa_K2268000) under the control of a LacI responsive promoter (BBa_K2268001). The latter two components act as an inverter: addition of IPTG will repress expression of the LacZ reporter, while a lack of IPTG will lead to expression.
LacZ alpha fragment can associate with the omega fragment, if present, to restore beta-galactosidase activity to a cell. This can be visualized as a blue pigment color when cells are grown in the presence of X-gal.
Note: the Sequence diagram below does not reflect the promoter, BBa_K2268001, that drives expression of the cI repressor (K2268000) for technical reasons.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]