Difference between revisions of "File:BBa Characterization LiU.jpg"
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| − | Characterization of BBa_K750000 with 6 different concentrations of arabinose (10, 1, 0.1, 0.01, 0.001, 0), compared to eGFP induced with a strong T7 promoter and empty BL21 (DE3) Codon+ cells. The measurements was done using an infinite M1000 pro plate reader at 37*C. Between measurements the plate was shaking with 216 rpm. Cells with BBa_K750000 were done in 12 replicates (4 technical and 3 biological (different clones from the same agarplate), the empty cells received the same amounts of arabinose as the cells with BBa_K750000 but were measured in technical duplicate (2 of each conc.) and the eGFP reference all were induced with 0.2 mM IPTG (12 technical replicates). | + | Characterization of BBa_K750000 with 6 different concentrations of arabinose (10, 1, 0.1, 0.01, 0.001, 0 mM), compared to eGFP induced with a strong T7 promoter and empty BL21 (DE3) Codon+ cells. The measurements was done using an infinite M1000 pro plate reader at 37*C. Between measurements the plate was shaking with 216 rpm. Cells with BBa_K750000 were done in 12 replicates (4 technical and 3 biological (different clones from the same agarplate), the empty cells received the same amounts of arabinose as the cells with BBa_K750000 but were measured in technical duplicate (2 of each conc.) and the eGFP reference all were induced with 0.2 mM IPTG (12 technical replicates). |
Latest revision as of 12:52, 27 October 2017
Characterization of BBa_K750000 with 6 different concentrations of arabinose (10, 1, 0.1, 0.01, 0.001, 0 mM), compared to eGFP induced with a strong T7 promoter and empty BL21 (DE3) Codon+ cells. The measurements was done using an infinite M1000 pro plate reader at 37*C. Between measurements the plate was shaking with 216 rpm. Cells with BBa_K750000 were done in 12 replicates (4 technical and 3 biological (different clones from the same agarplate), the empty cells received the same amounts of arabinose as the cells with BBa_K750000 but were measured in technical duplicate (2 of each conc.) and the eGFP reference all were induced with 0.2 mM IPTG (12 technical replicates).
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 12:41, 27 October 2017 |  | 3,366 × 1,500 (684 KB) | Momaxox (Talk | contribs) | Characterization of BBa_750000 with 6 different concentrations of arabinose (10, 1, 0.1, 0.01, 0.001, 0 mM), compared to eGFP induced with a strong T7 promoter and empty BL21 (DE3) Codon+ cells. The measurements was done using an infinite M1000 pro pla... |
| 12:27, 27 October 2017 |  | 3,366 × 1,668 (754 KB) | Momaxox (Talk | contribs) | Characterization of BBa_750000 with 6 different concentrations of arabinose (10, 1, 0.1, 0.01, 0.001, 0), compared to eGFP induced with a strong T7 promoter and empty BL21 (DE3) Codon+ cells. The measurements was done using an infinite M1000 pro plate... | |
| 10:00, 27 October 2017 | Error creating thumbnail: Invalid thumbnail parameters | 6,732 × 3,336 (1.78 MB) | Momaxox (Talk | contribs) | Characterization of BBa_750000 with 6 different concentrations of arabinose (10, 1, 0.1, 0.01, 0.001, 0), compared to eGFP induced with a strong T7 promoter and empty BL21 (DE3) Codon+ cells. The measurements was done using an infinite M1000 pro plate... |
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