Difference between revisions of "Part:BBa K2440036"
(→Usage and Biology) |
|||
| Line 8: | Line 8: | ||
MiRNA locker assembled by using this modularized DNA part was able to bind miR7514 in an Ago2 dependent manner, that is, knockdown of miR7514 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR7514 in an Ago2 dependent manner, that is, knockdown of miR7514 was achieved by transfecting cells with miRNA locker. | ||
| + | |||
| + | Ghr-miR7514 was firstly detected in in cotton ovules.1 | ||
| + | MiR7514 is found to be differentially expressed in PHYA1 RNAi cotton and it may be related with the fiber quality of cotton2. | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 02:05, 31 October 2017
miR7514 target sequence
It is the target sequence of miR7514, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR7514 in an Ago2 dependent manner, that is, knockdown of miR7514 was achieved by transfecting cells with miRNA locker.
Ghr-miR7514 was firstly detected in in cotton ovules.1 MiR7514 is found to be differentially expressed in PHYA1 RNAi cotton and it may be related with the fiber quality of cotton2.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.