Difference between revisions of "Part:BBa K2440031"

Revision as of 01:57, 31 October 2017

miR-155 target sequence

It is the target sequence of miR-155, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-155 in an Ago2 dependent manner, that is, knockdown of miR-155 was achieved by transfecting cells with miRNA locker.

Dre-miR155 was firstly found in zebrafish.1

MiR-155-5p are highly expressed in the heart and regulate cardiac development. What’s more, miR-155-5p was overexpressed in trisomic hearts2.

MiR-155 downregulation in NK cells of IA patients impaired IFN-γ production by targeting SOCS1, which may contribute to immune dysfunction during CHB infection. Additionally, baseline miR-155 expression could predict the treatment response to telbivudine therapy3.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

@@ Line 8: / Line 8: @@
 MiRNA locker assembled by using this modularized DNA part was able to bind miR-155 in an Ago2 dependent manner, that is, knockdown of miR-155 was achieved by transfecting cells with miRNA locker.
+Dre-miR155 was firstly found in zebrafish.1
+MiR-155-5p are highly expressed in the heart and regulate cardiac development. What’s more, miR-155-5p was overexpressed in trisomic hearts2.
+MiR-155 downregulation in NK cells of IA patients impaired IFN-γ production by targeting SOCS1, which may contribute to immune dysfunction during CHB infection. Additionally, baseline miR-155 expression could predict the treatment response to telbivudine therapy3.
 ===Sequence and Features===