Difference between revisions of "Part:BBa K2408023"

Revision as of 09:48, 29 October 2017

ADH1_Miraculin

This is a part improvment from BBa_K1033121 - team Uppsala 2013

Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag.

The miraculin glycoprotein, originally produced from the Synsepalum dulcificum plant, is an effectively non-cariogenic and acceptable for diabetics in flavor and sweetening formulations and has the ability to convert the sour taste to the sweet taste, and provides a sugar substitute because it has a glycemic index of zero. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of the special protein is still not entirely clear but it is known that in the presence of food / beverage products with a pH level between 3-6 factors trigger the reaction that will give the sense of sweetness effective.

We added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1110
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 771
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1222
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1110
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1110
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 93

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2408023 short</partinfo>
-'''This is a part improvment from BBa_K1033121 - team Uppsala 2013'''
+'''This is a part improvment from [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1033121 BBa_K1033121] - team Uppsala 2013'''
-Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag
+Miraculin optimized for <i>S. cerevisae</i> with the ADH1 promoter and histidine tag.
-The miraculin glycoprotein, originally produced from the Synsepalum dulcificum plant, is an effectively non-cariogenic and acceptable for diabetics in flavor and sweetening formulations and has the ability to convert the sour taste to the sweet taste, and provides a sugar substitute because it has a glycemic index of zero. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of the special protein is still not entirely clear but it is known that in the presence of food / beverage products with a pH level between 3-6 factors trigger the reaction that will give the sense of sweetness effective
+The miraculin glycoprotein, originally produced from the <i>Synsepalum dulcificum</i> plant, is an effectively non-cariogenic and acceptable for diabetics in flavor and sweetening formulations and has the ability to convert the sour taste to the sweet taste, and provides a sugar substitute because it has a glycemic index of zero. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of the special protein is still not entirely clear but it is known that in the presence of food / beverage products with a pH level between 3-6 factors trigger the reaction that will give the sense of sweetness effective.
-we added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.
+We added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.
 <!-- Add more about the biology of this part here