Difference between revisions of "Part:BBa K2492002"
| Line 8: | Line 8: | ||
b.some codons modified | b.some codons modified | ||
The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure). | The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure). | ||
| + | |||
| + | Universal mCherry coding sequence of three introns for capacity of expression in C.elegans. MCherry is a commonly used reporter. Since ordinary mCherry may not have high expression in C.elegans, artificial introns are inserted in the constructed plasmid[1]. We can obtain ord-10::Chrimson::GEM-GECO::mCherry transgenic offsprings microinjection. MCherry driven by promoter ord-10(Part:BBa_K2492004) indicates the position of neuron AWA. Moreover the fluorescence of mCherry aids the imaging for the neuron AWA pattern. The intensity of fluorescence reveals the expression level approximately. | ||
| + | [1]:S.Redemann. (2011). codon adaptation– based control of protein expression in C. elegans, Nature Methods, Vol.8 No.3 250-254. | ||
| + | |||
[[File: T--SUSTech_Shenzhen--C.elegans_AWA_mcherry.png|500px|thumb|left|mcherry expressed in AWA]] | [[File: T--SUSTech_Shenzhen--C.elegans_AWA_mcherry.png|500px|thumb|left|mcherry expressed in AWA]] | ||
Revision as of 15:23, 27 October 2017
MCherry optimized with three introns for C.elegans
To indicate neuron AWA in C.elegans’ head, we chose mCherry. We found that the part BBa_K2005050 is mCherry submitted by iGEM16_Vanderbilt. However, this mCherry is not designed for C.elegans. So, we got the sequence and improved it with C.elegans codon adaptor[1]. Compared with original mCherry, new synthesis mCherry sequence is modified in: a.adding three introns b.some codons modified The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure).
Universal mCherry coding sequence of three introns for capacity of expression in C.elegans. MCherry is a commonly used reporter. Since ordinary mCherry may not have high expression in C.elegans, artificial introns are inserted in the constructed plasmid[1]. We can obtain ord-10::Chrimson::GEM-GECO::mCherry transgenic offsprings microinjection. MCherry driven by promoter ord-10(Part:BBa_K2492004) indicates the position of neuron AWA. Moreover the fluorescence of mCherry aids the imaging for the neuron AWA pattern. The intensity of fluorescence reveals the expression level approximately. [1]:S.Redemann. (2011). codon adaptation– based control of protein expression in C. elegans, Nature Methods, Vol.8 No.3 250-254.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3