Difference between revisions of "Part:BBa K2492002"

Revision as of 11:39, 27 October 2017

MCherry optimized with three introns for C.elegans

To indicate neuron AWA in C.elegans’ head, we chose mCherry. We found that the part BBa_K2005050 is mCherry submitted by iGEM16_Vanderbilt. However, this mCherry is not designed for C.elegans. So, we got the sequence and improved it with C.elegans codon adaptor[1]. Compared with original mCherry, new synthesis mCherry sequence is modified in: a.adding three introns b.some codons modified The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure).

mcherry expressed in AWA

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2492002 short</partinfo>
-Mcherry, RFP, with three introns added. Mcherry is following the promoter ord-10, which can lead to high expression in AWA. We use this modified mcherry to locate the neuron AWA in C.elegans.
 To indicate neuron AWA in C.elegans’ head, we chose mCherry. We found that the part BBa_K2005050 is mCherry submitted by iGEM16_Vanderbilt. However, this mCherry is not designed for C.elegans.
 So, we got the sequence and improved it with C.elegans codon adaptor[1]. Compared with original mCherry, new synthesis mCherry sequence is modified in:
 a.adding three introns
 b.some codons modified
 The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure).