Difference between revisions of "Part:BBa K2492002:Experience"

Revision as of 17:28, 1 November 2017

Universal mCherry coding sequence of three introns for good expression in C.elegans. MCherry is a commonly used reporter. Since ordinary mCherry(part BBa_K2005050) may not have high expression in C.elegans, artificial introns are inserted in the constructed plasmid[1]. We can obtain ord-10::Chrimson::GEM-GECO::mCherry transgenic offsprings microinjection. MCherry driven by promoter ord-10(Part:BBa_K2492004) indicates the position of neuron AWA. Moreover the fluorescence of mCherry aids the imaging for the neuron AWA pattern. The intensity of fluorescence reveals the expression level approximately. The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure).

[1]:S.Redemann. (2011). codon adaptation– based control of protein expression in C. elegans, Nature Methods, Vol.8 No.3 250-254.

Applications of BBa_K2492002

mcherry expressed in AWA

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UNIQ1b9475d37c353499-partinfo-00000000-QINU UNIQ1b9475d37c353499-partinfo-00000001-QINU

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 [[File: T--SUSTech_Shenzhen--C.elegans_AWA_mcherry.png|500px|thumb|left|mcherry expressed in AWA]]
+{{SUSTech_Image | filename=T--SUSTech_Shenzhen--Microfuildics--cycle.gif|width=600px|caption=<B>Fig.1 Control the <i>C. elegans</i> by the light.</B> We used the optical fiber to form a light spot and the worm can follow the spot.}}
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