Difference between revisions of "Part:BBa K2448034"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | This is the original sequence of the D-Psicose 3-epimerase (Dpe) from ''Agrobacterium tumefaciens'' str. C58 (Atu4750, UniProt A9CH28, [[Part:BBa_K2448022|BBa_K2448022]]) equipped with a strong RBS ([[Part:BBa_K2448020|BBa_K2448020]]). | ||
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+ | ===Usage and Biology=== | ||
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+ | D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-psicose being the carbon-3 (C3) epimer of D-fructose. | ||
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+ | [[File: T--Evry_Paris-Saclay--EC 5.1.3.30.png|300px]] | ||
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+ | The ''A. tumefaciens'' Dpe is a 31.56 kDa protein of 289 amino acids. | ||
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+ | This enzyme is highly specific for D-Psicose, but it is also able to catalyse with very low activity the epimerization of other D-ketoses (in the decreased substrate specificity) : D-fructose, D-tagatose, D-ribulose, D-xylulose and D-sorbose [1]. | ||
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Revision as of 10:57, 1 November 2017
D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens
D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 208
Illegal NgoMIV site found at 268
Illegal NgoMIV site found at 619
Illegal AgeI site found at 646 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 174