Difference between revisions of "Part:BBa K2309022:Experience"

Revision as of 09:42, 1 November 2017

Applications of BBa_K2309022

This year we use dot blot method to evaluate part K2309022 work.

The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.

For LL-37 ability assay, we tested Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result is shown as follows:

As picture shows that the concentration of LL-37 from 5ug/ml to 10ug/ml, the LL-37 has highly activities Staphylococcus aureus.

We also test the ability of LL-37 attact biofilm of Staphylococcus aureus, the result as follows:

The result shows that the value of 595nm ABS is dicrease while the increase of LL-37 concentration. It seems that LL-37 peptide inhibite Staphylococcus aureus to form the biofilm.

User Reviews

UNIQ44def0b1378fdd69-partinfo-00000000-QINU UNIQ44def0b1378fdd69-partinfo-00000001-QINU

@@ Line 9: / Line 9: @@
 The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.
-For LL-37 ability assay, we test Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result as follows:
+For LL-37 ability assay, we tested Minimal Inhibitory concentration(MIC) of <i>Staphylococcus aureus</i>, the result is shown as follows:
 https://static.igem.org/mediawiki/parts/5/5c/LL-37_MIC_XJTLU-CHINA.jpeg
-As picture shows that the concentration of 5-10ug/ml, the LL-37 has highly activity.
+As picture shows that the concentration of LL-37 from 5ug/ml to 10ug/ml, the LL-37 has highly activities Staphylococcus aureus.
 We also test the ability of LL-37 attact biofilm of Staphylococcus aureus, the result as follows: