Difference between revisions of "Part:BBa K2244006"
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Co1E promoter is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription. | Co1E promoter is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Tomizawa, J. 1985. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible.Cell. 40(3):527-35. | Tomizawa, J. 1985. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible.Cell. 40(3):527-35. | ||
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Revision as of 07:13, 27 October 2017
ColE promoter
Co1E promoter is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.
Usage and Biology
Co1E promoter is 151bp in length. We used a light-repressed system containing a single light repressor LexA-VVD, and a ColE promoter that contains a SOS operator sequence for LexA binding to regulate the expression of target genes. The picture shows a colony PCR product of ColE promoter in pLEV1(408) vector.
Reference
Tomizawa, J. 1985. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible.Cell. 40(3):527-35.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]