Difference between revisions of "Part:BBa K2382012"
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<partinfo>BBa_K2382012 short</partinfo>
 
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A rigid linker link two proteins to form one fusion protein. It can prevent the
 
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two proteins from interrupting each other while folding.
 
  
 
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===Usage and Biology===
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==Usage and Biology==
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<span class='h3bb'>Sequence and Features</span>
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===<span class='h3bb'>Sequence and Features</span>===
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<partinfo>BBa_K2382012 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2382001 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2382012 parameters</partinfo>
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<partinfo>BBa_K2382001 parameters</partinfo>
 
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===Short Description===
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A rigid linker link two proteins to form one fusion protein. It can prevent the
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two proteins from interrupting each other while folding.
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 +
<!-- Add more about the biology of this part here
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===Usage and Biology===
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The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the
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target protein becomes automatically released from the fusion protein without proteolytic treatment.
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Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has
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been successfully employed for many cases of protein purification without the tag of a fusion protein.
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===References===
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Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated
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EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021

Revision as of 16:36, 27 October 2017

EAAAK

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Short Description

A rigid linker link two proteins to form one fusion protein. It can prevent the two proteins from interrupting each other while folding.

Usage and Biology

The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the target protein becomes automatically released from the fusion protein without proteolytic treatment. Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has been successfully employed for many cases of protein purification without the tag of a fusion protein.

References

Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021