Difference between revisions of "Part:BBa K2260000:Design"
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The naturally existing phaCAB operon is present in <i>R. eutropha</i> H16. The operon sequence was taken from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> and rearranged.</p> | The naturally existing phaCAB operon is present in <i>R. eutropha</i> H16. The operon sequence was taken from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> and rearranged.</p> | ||
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===References=== | ===References=== | ||
Revision as of 07:21, 30 October 2017
The phaCBA operon was codon optimized for use in E. coli using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from BBa_K1149051 were removed, including any new restriction sites introduced from codon optimization. Lastly, his tags were added to the operon.
===Source===The naturally existing phaCAB operon is present in R. eutropha H16. The operon sequence was taken from BBa_K1149051 and rearranged.