Difference between revisions of "Part:BBa K2260000:Design"
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===Design Notes=== | ===Design Notes=== | ||
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The phaCBA operon was codon optimized for use in <i>E. coli</i> using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, including any new restriction sites introduced from codon optimization. | The phaCBA operon was codon optimized for use in <i>E. coli</i> using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, including any new restriction sites introduced from codon optimization. | ||
</p> | </p> |
Revision as of 06:56, 27 October 2017
PhaCBA operon w/ Histidine tags
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The phaCBA operon was codon optimized for use in E. coli using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, including any new restriction sites introduced from codon optimization.
Source
Insert bacterial species' genome(s) here.