Difference between revisions of "Part:BBa K2260002"
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| − | Phasin is an intracellular protein native to PHB-producing bacteria, and binds to PHB deposits within the cell through electrostatic interaction. HlyA is a toxin produced by <i>E. coli<i> | + | Phasin is an intracellular protein native to PHB-producing bacteria, and binds to PHB deposits within the cell through electrostatic interaction. HlyA is a toxin produced by <i>E. coli</i> that is secreted via an endogenous, single-step type one secretion system. By adding the C-terminus of the HlyA gene to the end of the phasin molecule, the phasin is marked for secretion through the pathway designed for the HlyA. As a result, the phasin molecule binds to intracellular PHB granules and then is secreted due to the HlyA tag, effectively transporting PHB outside of the cell. |
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Revision as of 06:47, 27 October 2017
Phasin-HlyA
Phasin is an intracellular protein native to PHB-producing bacteria, and binds to PHB deposits within the cell through electrostatic interaction. HlyA is a toxin produced by E. coli that is secreted via an endogenous, single-step type one secretion system. By adding the C-terminus of the HlyA gene to the end of the phasin molecule, the phasin is marked for secretion through the pathway designed for the HlyA. As a result, the phasin molecule binds to intracellular PHB granules and then is secreted due to the HlyA tag, effectively transporting PHB outside of the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]