Difference between revisions of "Part:BBa K2333413:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
considerations
+
This part was designed with a constitutive promoter for the mScarlet-I reporter as a tagless control construct.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
nature paper  
+
UNS sequences are from Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly.
 +
 
 +
The sequence for mScarlet-I was obtained and modified from the sequence in the paper by Bindels et al. "2016 MScarlet: a bright monomeric red fluorescent protein for cellular imaging." The sequence was modified to remove the start codon.
  
 
===References===
 
===References===
 +
 +
[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., . . . Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074
 +
 +
[2] Cameron DE, Collins JJ. Tunable protein degradation in bacteria. Nature Biotechnology. 2014;32(12):1276–1281.
 +
 +
[3] Torella JP, Boehm CR, Lienert F, Chen J-H, Way JC, Silver PA. Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic Acids Research. 2013;42(1):681–689.

Revision as of 21:33, 29 October 2017


UNS J23100 mScarlet-I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
    Illegal NotI site found at 605
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed with a constitutive promoter for the mScarlet-I reporter as a tagless control construct.

Source

UNS sequences are from Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly.

The sequence for mScarlet-I was obtained and modified from the sequence in the paper by Bindels et al. "2016 MScarlet: a bright monomeric red fluorescent protein for cellular imaging." The sequence was modified to remove the start codon.

References

[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., . . . Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074

[2] Cameron DE, Collins JJ. Tunable protein degradation in bacteria. Nature Biotechnology. 2014;32(12):1276–1281.

[3] Torella JP, Boehm CR, Lienert F, Chen J-H, Way JC, Silver PA. Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic Acids Research. 2013;42(1):681–689.