Difference between revisions of "Part:BBa K2364042"

 
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<partinfo>BBa_K2364042 short</partinfo>
 
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        Supplementary Experiment
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        <p>Group: CSU_CHINA 2021
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        <p>Author: Xingjun Zhao
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        <p>T21 is called miR21T in our project, it is a DNA sequence that can be the target of miRNA21. We have enhanced the method used by iGEM17_Greece in 2017 and inserted a piece of T21 in target gene 5’UTR and 4 pieces of T21s in 3’UTR, significantly
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            improved the efficiency of miR21 targeting T21 and suppressing the expression of target gene, meet our feedback suppression requirement in our loop.
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            <img width="400px" src="https://2021.igem.org/wiki/images/8/86/T--CSU_CHINA--tupian59.png"></p>
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        <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 Electrophoretic diagram of miR21T-LUC-4XmiR21T PCR product</p>
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        <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 miR21 inhabited the expression of reporter LUC</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:52, 20 October 2021

Τ21

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Supplementary Experiment

Group: CSU_CHINA 2021

Author: Xingjun Zhao

T21 is called miR21T in our project, it is a DNA sequence that can be the target of miRNA21. We have enhanced the method used by iGEM17_Greece in 2017 and inserted a piece of T21 in target gene 5’UTR and 4 pieces of T21s in 3’UTR, significantly improved the efficiency of miR21 targeting T21 and suppressing the expression of target gene, meet our feedback suppression requirement in our loop.

Fig.1 Electrophoretic diagram of miR21T-LUC-4XmiR21T PCR product

Fig.2 miR21 inhabited the expression of reporter LUC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]