Difference between revisions of "Part:BBa I751021"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_I751021 short</partinfo> | <partinfo>BBa_I751021 short</partinfo> | ||
+ | This is made for A-side plasmid in our project. | ||
+ | |||
+ | Regulated by lac repressor, this part makes LuxI, cI repressor, and mCherry (for detecting). | ||
+ | If needed, you can move this part into B-side plasmid (by DraIII or AatII), regulating the rate of cI/lac repressor. | ||
+ | This part is for our second plan, in case BBa_I751011 doesn't work. | ||
Revision as of 17:26, 24 October 2007
plac-lux-cI-mCherry This is made for A-side plasmid in our project.
Regulated by lac repressor, this part makes LuxI, cI repressor, and mCherry (for detecting).
If needed, you can move this part into B-side plasmid (by DraIII or AatII), regulating the rate of cI/lac repressor.
This part is for our second plan, in case BBa_I751011 doesn't work.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 2762
Illegal suffix found in sequence at 5246 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2762
Illegal SpeI site found at 5247
Illegal PstI site found at 5261
Illegal NotI site found at 2768
Illegal NotI site found at 5254 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2762
Illegal BglII site found at 3016
Illegal BglII site found at 3698
Illegal BamHI site found at 2798 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2762
Illegal suffix found in sequence at 5247 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2762
Illegal XbaI site found at 2777
Illegal SpeI site found at 5247
Illegal PstI site found at 5261 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2003
Illegal SapI site found at 920