Difference between revisions of "Part:BBa K2440014"

Revision as of 01:00, 31 October 2017

miR-195 target sequence

It is the target sequence of miR-195, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-195 in an Ago2 dependent manner, that is, knockdown of miR-195 was achieved by transfecting cells with miRNA locker.

Hsa-miR-195 was firstly identified in lung, skin and kidney of mouse by cloning from mouse tissues.1

MicroRNA-195 has been reported as a cancer-related microRNA in many human cancers. Researchers find that miR-195 directly targets the fatty acid synthase enzyme and negatively regulates the expression of fatty acid synthase and finally demonstrated that MiR-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase2.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

@@ Line 8: / Line 8: @@
 MiRNA locker assembled by using this modularized DNA part was able to bind miR-195 in an Ago2 dependent manner, that is, knockdown of miR-195 was achieved by transfecting cells with miRNA locker.
+Hsa-miR-195 was firstly identified in lung, skin and kidney of mouse by cloning from mouse tissues.1
+MicroRNA-195 has been reported as a cancer-related microRNA in many human cancers. Researchers find that miR-195 directly targets the fatty acid synthase enzyme and negatively regulates the expression of fatty acid synthase and finally demonstrated that MiR-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase2.
 ===Sequence and Features===