Difference between revisions of "Part:BBa K2440012"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker. | ||
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| + | Hsa-miR-4470 is one of the founding members of the miRNA family. It was first found in normal and malignant human B-cells1.And it is located in 8q12.3.Its function hasn't been researched yet. | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 00:56, 31 October 2017
miR-4470 target sequence
It is the target sequence of miR-4470, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker.
Hsa-miR-4470 is one of the founding members of the miRNA family. It was first found in normal and malignant human B-cells1.And it is located in 8q12.3.Its function hasn't been researched yet.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.