Difference between revisions of "Part:BBa K2477009"

 
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<partinfo>BBa_K2477009 short</partinfo>
 
<partinfo>BBa_K2477009 short</partinfo>
  
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This TLP coding region contains two tags. The first is a 6x His-tag for easy protein isolation. The second is an HlyA tag which will allow the protein to be exported from the cell via the HlyBD complex. The two tags are connected via a 2x GS linker.
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This TLP coding region contains a 6x His-tag for easy followed by a 2x GS linker on the C-terminus. This is not an actual basic part, but rather a part stored in the system so as to be combined properly with BBa_K1166002 in another composite part so as to generate the correct sequence.
  
 
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Latest revision as of 22:38, 26 October 2017


TLP with 6x His-tag 2x GS linker and HLYA tag

This TLP coding region contains a 6x His-tag for easy followed by a 2x GS linker on the C-terminus. This is not an actual basic part, but rather a part stored in the system so as to be combined properly with BBa_K1166002 in another composite part so as to generate the correct sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 120
    Illegal AgeI site found at 223
    Illegal AgeI site found at 265
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 407