Difference between revisions of "Part:BBa K2292004"
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K2292003 is the part that we used to detect one of our target molecule, CSP. It will produce membrane receptor, comD. ComD then will bind to CSP. The comD-CSP complex will phosphorylate comE. The phophorylated comE, finally will bind to a comE responsive promoter( on this part) and produce GFP. Through GFP intensity, we will learn the concentration of CSP.
 
K2292003 is the part that we used to detect one of our target molecule, CSP. It will produce membrane receptor, comD. ComD then will bind to CSP. The comD-CSP complex will phosphorylate comE. The phophorylated comE, finally will bind to a comE responsive promoter( on this part) and produce GFP. Through GFP intensity, we will learn the concentration of CSP.
  
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https://i.imgur.com/AjavUGq.jpg
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<img src="https://static.igem.org/mediawiki/parts/a/ac/TCSTS~23~.jpg"  style="display:block; margin:auto;">
  
 
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Revision as of 22:38, 1 November 2017


CSP detection through GFP intensity

https://parts.igem.org/Part:BBa_K2292003

Our project aims to detect the risk of caries through three parameters: Competence stimulating peptide, CSP concentration, lactate level, pH value.

K2292003 is the part that we used to detect one of our target molecule, CSP. It will produce membrane receptor, comD. ComD then will bind to CSP. The comD-CSP complex will phosphorylate comE. The phophorylated comE, finally will bind to a comE responsive promoter( on this part) and produce GFP. Through GFP intensity, we will learn the concentration of CSP.

<img src="TCSTS~23~.jpg" style="display:block; margin:auto;">

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 786