Difference between revisions of "Part:BBa K2292005"
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This construction is cloned into E.coli. | This construction is cloned into E.coli. | ||
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O1 and O2 binding is added on two sides of promoter(J23118) | O1 and O2 binding is added on two sides of promoter(J23118) | ||
Revision as of 22:49, 1 November 2017
Lactate detection
Our project aims to detect the risk of caries through three parameters: Competence stimulating peptide, CSP concentration, lactate level, pH value.
This construction is used for lactate quantization We made use of the property of LldR operon. The LldR is transcription factor. Without lactate, it will bind on O1&O2 operator site, form a DNA loop, blocking the transcription. As lactate present, lactate will bind on LldR and open the loop, allowing the transcription to go on and produce GFP. GFP intensity is then detected and deduct lactate level. This construction is cloned into E.coli.
O1 and O2 binding is added on two sides of promoter(J23118)
Sequence:
O1: AATTGGCCCTACCAATT O2: AATTGGCAGTGCCACTT
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 993
Illegal NheI site found at 1016 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 628
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1691