Difference between revisions of "Part:BBa K2206011"
| Line 5: | Line 5: | ||
Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region. | Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region. | ||
| − | The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch. | + | The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch. The formation of the duplex occurs through a highly specific binding step which results in single-base mismatch specificity. |
The trigger RNA sequence for BBa_K2206010 is divided between hsa-miR-27b-3p and an anti-miRNA. This part is the anti-miRNA required for complete trigger RNA formation and activation of BBa_K2206010. This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-27b-3p and one which is complimentary to the 5' end of BBa_K2206010. | The trigger RNA sequence for BBa_K2206010 is divided between hsa-miR-27b-3p and an anti-miRNA. This part is the anti-miRNA required for complete trigger RNA formation and activation of BBa_K2206010. This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-27b-3p and one which is complimentary to the 5' end of BBa_K2206010. | ||
Revision as of 19:02, 26 October 2017
Anti-miRNA for hsa-miR-27b-3p
Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.
The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch. The formation of the duplex occurs through a highly specific binding step which results in single-base mismatch specificity.
The trigger RNA sequence for BBa_K2206010 is divided between hsa-miR-27b-3p and an anti-miRNA. This part is the anti-miRNA required for complete trigger RNA formation and activation of BBa_K2206010. This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-27b-3p and one which is complimentary to the 5' end of BBa_K2206010.
For more information, see the section on second series of toehold switches on the design page of CLSB-UK's 2017 wiki - http://2017.igem.org/Team:CLSB-UK/Design
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]