Difference between revisions of "Part:BBa K2382001:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression.
+
It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression.  
 +
Otherwise, we remove the stop coden of NC_008596.1
  
 
===Source===
 
===Source===

Revision as of 19:03, 26 October 2017


MSMEG_5998


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression. Otherwise, we remove the stop coden of NC_008596.1

Source

https://www.ncbi.nlm.nih.gov/nuccore/118467340/


References

Lapalikar, G.V., et al., F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the Actinomycetales. PLoS One, 2012. 7(2): p. e30114.

Taylor, M.C., et al., Identification and characterization of two families of F420H2‐dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Molecular microbiology, 2010. 78(3): p. 561-575.