Difference between revisions of "Part:BBa K2235009"

Revision as of 15:37, 26 October 2017

Sialidase composite with T7 promoter and RBS

Introduction

This biobrick is a constitute of T7 promoter and RBS followed by sialidase enzyme coding site. Sialidase enzyme has the potential to digest terminal sialic acids in a glycoprotein. The sequence originates from the species Arthrobacter Ureafaciens.

Molecular cloning

Ligation of sialidase composite insert into pSB1C3

The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the double digestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.

Figure 1: From left to right: M DNA ladder, next 2 lanes are plasmids digested with Ecor1 and Pst1.

Important Parameters

Sequence and Features