Difference between revisions of "Part:BBa K2267040:Design"
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===Design Notes=== | ===Design Notes=== | ||
We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here | We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here | ||
| − | + | This part does not include an LVA tag on the LuxR gene. This means that the protein is not rapidly degraded. When active, the LuxR protein binds to the pLux promoter, activating transcription of downstream genes. This part contains everything necessary for O3C6-HSL-induced expression of genes inserted downstream of the pLux promoter. | |
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===Source=== | ===Source=== | ||
Revision as of 19:23, 1 November 2017
LuxR-I-Plux-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 837
Illegal NheI site found at 860 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 928
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1452
Illegal BsaI.rc site found at 2179
Design Notes
We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here This part does not include an LVA tag on the LuxR gene. This means that the protein is not rapidly degraded. When active, the LuxR protein binds to the pLux promoter, activating transcription of downstream genes. This part contains everything necessary for O3C6-HSL-induced expression of genes inserted downstream of the pLux promoter.
Source
its come from imperial