Difference between revisions of "Part:pSB6A1:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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<I>Username</I> | <I>Username</I> | ||
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| − | + | This plasmid has been adapted to the version 5 of BioBrick vector backbones by the ETHZ 2011 iGEM team. This means, that the cassettes for the ORI and antibiotic resistance have been minimized (reduction of the size and transcriptional terminators flanking the prefix and the suffix have been added, to trancriptionally insulate the inserted parts from the vector backbone machinery and vice versa. | |
| + | This minimization yielded a reduction in size from 4022 bp to 2743 bp. | ||
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| + | For comparison of the copy number of the plasmids, triplicates of OD normalized bacterial cultures containing the respective plasmids were miniprepped and the DNA concentration determined by an agarose DNA gel of a dilution series of an EcoRI digest. | ||
| + | This experiment yielded a reduction in copy number of | ||
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|}; | |}; | ||
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Revision as of 15:41, 1 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of pSB6A1
User Reviews
UNIQ8303ef62c5e28b97-partinfo-00000000-QINU UNIQ8303ef62c5e28b97-partinfo-00000001-QINU