Difference between revisions of "Part:BBa K2398555:Design"

 
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===Design Notes===
 
===Design Notes===
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As it is only a 54 base pair long nucleotide sequence it is recommended to oligo anneal this part with sticky ends and use it in a golden gate assemble reaction.  
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This part is only 54 base pair long nucleotide sequence. Therefore it is recommended to order two complementary oligos with overhangs corresponding to restriction overhangs used in golden gate assembly. This is also the reason for not submitting this part. To functionalize this part you have to anneal both oligos in a Touchdown-PCR approach.
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===Source===
 
===Source===

Latest revision as of 14:33, 27 October 2017


Organosilicon binding Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is only 54 base pair long nucleotide sequence. Therefore it is recommended to order two complementary oligos with overhangs corresponding to restriction overhangs used in golden gate assembly. This is also the reason for not submitting this part. To functionalize this part you have to anneal both oligos in a Touchdown-PCR approach.

Source

This part has no source whatsoever. It is derived from in silico models for the binding of organosilicons.

References