Difference between revisions of "Part:BBa K2292001"
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ComE is a response regulator of competence regulon. It binds to comD-CSP complex be phosphorylated. The phosphorylated comE will bind onto a comE responsive promoter on downstream gene, turn on the transcription of it. | ComE is a response regulator of competence regulon. It binds to comD-CSP complex be phosphorylated. The phosphorylated comE will bind onto a comE responsive promoter on downstream gene, turn on the transcription of it. | ||
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| + | In our project, we replaced the downstream gene with GFP. Below is a picture showing how this part work in our construction | ||
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| + | https://i.imgur.com/mG1Bzkr.jpg | ||
Quorum sensing (QS), is a methodology of bacteria to communicate, just like language to us human. The bacteria learn their population situation by accepting or delivering quorum sensing molecules. Quorum sensing can work on bacteria of the same species and bacteria of different species as well. They can interact with the very specific molecule within same species just like native language; and interact with universal molecule inter-species, just like a universal foreign language. | Quorum sensing (QS), is a methodology of bacteria to communicate, just like language to us human. The bacteria learn their population situation by accepting or delivering quorum sensing molecules. Quorum sensing can work on bacteria of the same species and bacteria of different species as well. They can interact with the very specific molecule within same species just like native language; and interact with universal molecule inter-species, just like a universal foreign language. | ||
Revision as of 14:34, 26 October 2017
Response regulator of competence regulon, comE
ComE is a response regulator of competence regulon. It binds to comD-CSP complex be phosphorylated. The phosphorylated comE will bind onto a comE responsive promoter on downstream gene, turn on the transcription of it.
In our project, we replaced the downstream gene with GFP. Below is a picture showing how this part work in our construction
Quorum sensing (QS), is a methodology of bacteria to communicate, just like language to us human. The bacteria learn their population situation by accepting or delivering quorum sensing molecules. Quorum sensing can work on bacteria of the same species and bacteria of different species as well. They can interact with the very specific molecule within same species just like native language; and interact with universal molecule inter-species, just like a universal foreign language.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 164
Illegal XhoI site found at 19
Illegal XhoI site found at 49 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]