Difference between revisions of "Part:BBa K2407303"
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A composite part of three key genes to produce β-carotene which can be expressed in Saccharomyces Cerevisiae. | A composite part of three key genes to produce β-carotene which can be expressed in Saccharomyces Cerevisiae. | ||
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| + | ==Introduction== | ||
| + | After reviewing the literature, we understand that carotene can be overexpressed in Saccharomyces cerevisiae. Furthermore, carotenoid production levels were higher in strains containing integrated carotenogenic genes. Overexpression of crtYB and crtI from X. dendrorhous was sufficient to enable carotenoid production. crtYB, crtI, and crtE can complete the expression of β-carotene. | ||
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Revision as of 16:51, 26 October 2017
β-carotene gene
A composite part of three key genes to produce β-carotene which can be expressed in Saccharomyces Cerevisiae.
Introduction
After reviewing the literature, we understand that carotene can be overexpressed in Saccharomyces cerevisiae. Furthermore, carotenoid production levels were higher in strains containing integrated carotenogenic genes. Overexpression of crtYB and crtI from X. dendrorhous was sufficient to enable carotenoid production. crtYB, crtI, and crtE can complete the expression of β-carotene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 749
Illegal BglII site found at 2436
Illegal XhoI site found at 448
Illegal XhoI site found at 580
Illegal XhoI site found at 1399 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 616
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3340
Illegal BsaI site found at 5695
Illegal BsaI.rc site found at 176
Illegal BsaI.rc site found at 6026
Illegal SapI site found at 2740