Difference between revisions of "Part:BBa K2285013"
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constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilGFP as the reporter gene. The original part of amilGFP chromoprotein is presented on <partinfo>BBa_K592010</partinfo>. What should be noticed is, the original part is a coding part and doesn't have its own RBS and Term. Therefore, to achieve greater convenience fo our Star-chromoprotein reporter system, we add the RBS which is on the upstream of sfGFP(<partinfo>BBa_K515005</partinfo>) as well as a short terminator B1006 to all chromoproteins we used in this project via PCR. | constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilGFP as the reporter gene. The original part of amilGFP chromoprotein is presented on <partinfo>BBa_K592010</partinfo>. What should be noticed is, the original part is a coding part and doesn't have its own RBS and Term. Therefore, to achieve greater convenience fo our Star-chromoprotein reporter system, we add the RBS which is on the upstream of sfGFP(<partinfo>BBa_K515005</partinfo>) as well as a short terminator B1006 to all chromoproteins we used in this project via PCR. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | More infomation and details about usage of this part can refer to '''J23119+Target3+eforRed''' <partinfo>BBa_K2285012</partinfo> | ||
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Revision as of 13:20, 26 October 2017
J23119+Target3+amilGFP(with RBS and Term added)
constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilGFP as the reporter gene. The original part of amilGFP chromoprotein is presented on BBa_K592010. What should be noticed is, the original part is a coding part and doesn't have its own RBS and Term. Therefore, to achieve greater convenience fo our Star-chromoprotein reporter system, we add the RBS which is on the upstream of sfGFP(BBa_K515005) as well as a short terminator B1006 to all chromoproteins we used in this project via PCR.
Usage and Biology
More infomation and details about usage of this part can refer to J23119+Target3+eforRed BBa_K2285012
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]