Difference between revisions of "Part:BBa I746001"
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<partinfo>BBa_I746001 short</partinfo> | <partinfo>BBa_I746001 short</partinfo> | ||
− | This part is simply the AIP generator ([https://parts.igem.org/wiki/index.php/Part:BBa_I746000 | + | This part is simply the AIP generator ([https://parts.igem.org/wiki/index.php/Part:BBa_I746000 BBa_I746000]) from the <i>agr</i> system with a standard registry terminator ([https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]) downstream. |
The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. | The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. |
Latest revision as of 21:42, 21 October 2021
I746000 (AIP generator - agrB + agrD with RBSes) + B0015 (Terminator)
This part is simply the AIP generator (BBa_I746000) from the agr system with a standard registry terminator (BBa_B0015) downstream.
The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.
In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.
This part contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a SaII site.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]