Difference between revisions of "Part:BBa K2520023"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2520023 short</partinfo> | <partinfo>BBa_K2520023 short</partinfo> | ||
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EF1a promoter | EF1a promoter | ||
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===Results=== | ===Results=== | ||
In order to test our mutant promoter we created, we tested the expression of the reporter gene GFP under three different promoters- CMV, EF1a native and the mutant EF1a we created. As shown in the following graph, the expression levels of the reporter gene under EF1a promoter was much higher comparing to the CMV promoter, and these levels are almost equal between the native and the mutant promoter. | In order to test our mutant promoter we created, we tested the expression of the reporter gene GFP under three different promoters- CMV, EF1a native and the mutant EF1a we created. As shown in the following graph, the expression levels of the reporter gene under EF1a promoter was much higher comparing to the CMV promoter, and these levels are almost equal between the native and the mutant promoter. | ||
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| + | <partinfo>BBa_K2520023 parameters</partinfo> | ||
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Revision as of 12:36, 26 October 2017
EF1a promoter
EF1a promoter
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 643
Illegal XhoI site found at 1042 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 777
Illegal AgeI site found at 151 - 1000COMPATIBLE WITH RFC[1000]